MAGeCK
Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout

About

Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK) is a computational tool to identify important genes from the recent genome-scale CRISPR-Cas9 knockout screens (GeCKO) technology.

Author

MAGeCK is developed and maintained by Wei Li and Han Xu from Dr. Xiaole Shirley Liu's lab at Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard School of Public Health.

Source code

The MAGeCK source code and documentation can be found on http://mageck.sourceforge.net.

Dataset

The sgRNA read count tables of the recently published GeCKO experiments can be downloaded as follows. After installing the mageck software, you can run the "test" command directly on these tables.

Dataset
Reference
Download link
Leukemia (by courtesy of Tim Wang)
Genetic Screens in Human Cells Using the CRISPR-Cas9 System.
leukemia.csv.zip
Melanoma
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.
(data extracted from genome-engineering.org)
melanoma.csv.zip
ESC
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library.
(data extracted from the Fig. 4 of the paper)
esc.csv.zip
 

Links

Liu Lab